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Gene name
|
Direction
|
Primer sequence (5′ -3′)a
|
|---|
|
tbe1_promoter_XmaI
|
Forward
|
ACTGCCCGGGAGGTCTCACGGATTCAGGAA
|
|
tbe1_XbaI
|
Reverse
|
ACTCTCTAGATCATTGCAGCATCCATTTAA
|
|
tbe1_promoter_XbaI
|
Forward
|
ACTCTCTAGATTGTAAATAACTCAGATTTGGT
|
|
tbe1_protmoter_overlapped
|
Reverse
|
AATTTTTTTCATATTTTCCTCCTGGTATTGCG
|
|
tbe2_overlapped
|
Forward
|
CAGGAGGAAAATATGAAAAAAATTCTAATCCT
|
|
tbe2_PstI
|
Reverse
|
ACTCCTGCAGTTATTTCTTGAAAAGTTGAG
|
- a Restriction enzyme recognition sequences and overlapped sequences are underlined
