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Table 3 Bacterial strains and plasmids used in this study

From: An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases

Strains and plasmids Relevant characteristic Source
E. coli strains
 DH10B pIndigoBAC-5 without inserted gene Laboratory collection
 BAC library DH10B, pIndigoBAC-5 harboring BALB/c mouse gut microbiome DNA [23]
 tEc wild type strain isolated from CD-1 mouse intestines [31]
 tbe1 tEc, pBAD24::tbe1 This study
 tbe2 tEc, pBAD24::tbe2 This study
 L. lactis Type strain KCTC 3619 Laboratory collection
 S. pneumoniae Type strain ATCC 49619 Laboratory collection
Plasmids
 pIndigoBAC-5 Cpr, library backbone vector Laboratory collection
 pBAD24 Ampr, cloning vector Laboratory collection
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Journal of Biological Engineering

ISSN: 1754-1611

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