Fig. 1

Schematic for sandwich detection of yeast surface-displayed Fn3 binding to Zika virions. Anti-Zika human IgG conjugated with Alexa488 enables detection of Fn3-Zika virion binding interaction. Alexa 405-conjugated anti-myc antibody (IgY from chicken) binds to myc tag on Fn3 C-terminus and allows quantification of Fn3 surface display