Table 2 Primer sequences for real time-PCR
From: CD24 identifies nucleus pulposus progenitors/notochordal cells for disc regeneration
| Gene | Primer sequence(5′- 3′) | |
|---|---|---|
| BRACHYURY | FORWARD | CTGCAGTACCGAGTGGATCA |
| REVERSE | ACAGCTCGCTCTCTTCCAGA | |
| GLUT-1 | FORWARD | ACGGTGCTGCTGGTACTCTT |
| REVERSE | TCAGGTGTCTTGTCGCTCTG | |
| SHH | FORWARD | ATGAACCAGTGGCCTGGA |
| REVERSE | CCTGTCAGACGTGGTGATGT | |
| KRT-8 | FORWARD | CAGGAGCTGATGAACGTCAA |
| REVERSE | TTCGTGTGGATGCTCATGTT | |
| KRT-18 | FORWARD | GCTGAGACCACACTCTTGGAG |
| REVERSE | TGTATCTGGCCTCCACTTCC | |
| NOTCH1 | FORWARD | TCGTGCTCCTGTTCTTTGTG |
| REVERSE | TTCTCTCCGCTTCTTCTTGC | |
| JAGGED-1 | FORWARD | CTGAGGACTACGAGGGCAAG |
| REVERSE | CCCTTCAGGAGTATCGTTGG | |
| HES-1 | FORWARD | CCCGTCTACCTCTCTCCTTG |
| REVERSE | TCCCCTTTACTTGGCTTTCA | |
| HIF-1α | FORWARD | TCAAGTCAGCAACGTGGAAG |
| REVERSE | TTCACAAATCAGCACCAAGC | |
| RUNX2 | FORWARD | CCACCACTCACTACCACACG |
| REVERSE | GGACGCTGACGAAGTACCAT | |
| OSTEOCALCIN | FORWARD | CCTGACTGCATTCTGCCTCT |
| REVERSE | AGGTAGCGCCGGAGTCTATT | |
| BMP-2 | FORWARD | GAAGCCAGGTGTCTCCAAGA |
| REVERSE | GGATGTCCTTTACCGTCGTG | |
| ALP | FORWARD | GACAAGAAGCCCTTCACAGC |
| REVERSE | ACTGGGCCTGGTAGTTGTTG | |
| ADIPONECTIN | FORWARD | ACCCAAGGAAACTTGTGCAG |
| REVERSE | CGTCTCCCTTCTCTCCCTTC | |
| C/EBPα | FORWARD | GCCAAGAAGTCGGTGGATAA |
| REVERSE | AACACCTTCTGCTGCGTCTC | |
| PPAR-γ | FORWARD | CGAGAAGGAGAAGCTGTTGG |
| REVERSE | TCAGCGGGAAGGACTTTATG | |
| LPL | FORWARD | CAGCTGGGCCTAACTTTGAG |
| REVERSE | GGATCCCAATACTTCGACCA | |
| SOX-9 | FORWARD | TCAACGGCTCCAGCAAGAACAAG |
| REVERSE | CTCCGCCTCCTCCACGAAGG | |
| COL-II | FORWARD | CTCAAGTCGCTGAACAACCA |
| REVERSE | GTCTCCGCTCTTCCACTCTG | |
| β-ACTIN | FORWARD | ACGGGAATGTGAAGATGACC |
| REVERSE | CTCTGACACCACAGGAGCAA |