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Lag between vaccine and lethal virus addition a
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Fold drop in concentration/titer of vaccine-infected cells due to T5 killing b
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no vaccine
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4500, 15550
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3 hr
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1692
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7 hr
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283
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overnight
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4, 37
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aCells at 108/mL were infected with vaccine and helper; the vaccine multiplicity of infection was 1, so infections by vaccine would thus have been nearly complete within 30 minutes
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bCounts to establish the baseline, before T5 addition, were of vaccine-infected (ampicillin-resistant) cells without specific regard to T5 sensitivity. (For ’no vaccine,’ initial counts are based on total cells.) At 3-4 hr after T5 addition (at the time of plating), T5 density was at least 10X that of cell density, so nearly all sensitive cells would have been been unable to form a colony
