You are viewing the site in preview mode
Skip to main content
|
Strain, Selection plate
|
QuickStep-Cloning
|
RF Cloning
|
Transformation efficiency [cfu/μg]
|
|---|
|
DH5α, Kanamycin
|
334
|
26
|
3.8 · 104
|
|
C41(DE3), Kan + IPTG
|
418(404)
|
113(103)
|
4.2 · 106
|
- Colony counts for E. coli strains DH5α and C41 (DE3) transformed with the products of RF cloning and of QuickStep-Cloning and plated on agar plates supplemented with: (i) 50 μg/ml kanamycin and (ii) 50 μg/ml kanamycin and 1 mM IPTG. Transformation efficiency was determined based on concurrent transformation of 1 ng intact pET24a-HLTEV-p53 plasmid. Numbers in the brackets denote RFP-expressing colonies, as determined by visual inspection of the plates
